|
HU Credits:
6
Degree/Cycle:
2nd degree (Master)
Responsible Department:
Bio-Medical Sciences
Semester:
1st and/or 2nd Semester
Teaching Languages:
English and Hebrew
Campus:
Ein Karem
Course/Module Coordinator:
Prof Yael Stern-Bach
Dr. Amalia Tabib
Coordinator Office Hours:
scheduled by Email
Teaching Staff:
Prof Yael Stern-Bach,
Dr. Amalia Tabib,
Prof Ayal Ben-Zvi,
Prof Michael Berger,
Dr. Shaya Lev,
Dr. Ori Shalev,
Dr. Shai Sabbah,
Prof Yossi Buganim,
Prof Asaf Hellman,
Prof Dana Reichmann
Course/Module description:
This will primarily be a training course for master student indented to open the first year of master in which the students will be introduced to various biomedical research approaches and tools. This will include introduction to instruments in the faculty core facility. Students will learn the theories of the approaches and their applications in research. While each student starts master studies with different backgrounds, they will be able to translate knowledge established during their bachelor’s into practical methodologies for their master training. In addition this course will include introduction to the faculty research infrastructure, overview of research approaches in their future labs and that of other groups in the faculty.
The course will be conducted in collaboration with the the Core Research Facility of the Faculty.
Course/Module aims:
To expose students to various scientific approaches, methods and instruments and their applications in biomedical research. Mastering both theories of the approaches and their applications in research.
Learning outcomes - On successful completion of this module, students should be able to:
To evaluate research questions in light of advance research approaches.
To plan scientific system by using different research approaches.
To master both theories and practical applications of each approach.
To know the faculty research infrastructure, the core facility team and researchers in the faculty which are expert in different approaches.
All these will serve as future guidance for the student’s future research.
Attendance requirements(%):
100
Teaching arrangement and method of instruction:
Lectures, assignments, lab work, meetings with heads of research infrastructure units at the core facility and instruments demonstrations.
Course/Module Content:
Imaging approaches and microscopy:
• Introduction:
Electromagnetic spectrum
The light wave characters: wavelength & energy, Amplitude, phase
Introduction to imaging infrastructure including light microscopy unit, EM unit, image analysis service, spectrophotometric and the imaging center for pre-clinical animal model (including PET/CT/MRI/ultrasound).
Light microscope:
Bright field
Phase contrast
• Fluorescent microscope:
Fluorescent microscope
Confocal microscope
Time lapse microscopy
Super Resolution – STORM
Super Resolution – STED
• Electron Microscope:
Transmission Electron Microscope (TEM)
Scanning Electron Microscope (SEM)
Flow cytometry & FACS
Principle of operation: FACS components (laser, optics, flow, and types of detectors and their location).
Type signals received: Area, Width and Height.
Protocols, applications, analysis of results and presentation. Introduction to Flow Cytometry unit with FACS, Analyzers and Imagestream.
• Sequencing
Sanger, next generation sequencing
Introduction to Single cell technologies
In between single cell FACS sorting and genomic applications, single cell technologies will be introduced with additional sorting approaches such as microfluidics (10X Genomics) etc.
Molecular Biology
PCR, qPCR
•KD
shRNA, siRNA, miRNA
•KO, KI
Classical, crispr
Induced KO – cre-loxp, ER
Crispr as a tool for Activation of genes.
Introduction to the GEMM unit.
• Epigenetics:
ChIP, Bisulfite, RRBS
• Genomics
Chromosome Conformation Capture- HiC, 4C, 3C
Introduction to the Genomic application unit.
• Proteins
Western blot
Co-IP
MassSpec
Introduction to the Proteomic/metabolomics unit.
Required Reading:
1. Molecular Biology of The Cell, Alberts et al., 6th ed. 2014-
2. Essential Cell Biology, Alberts et al., 5nd Ed. 2019.
3. Molecular Cell Biology, Lodish et al, 8th Ed, 2016
4. Genes XI, Lewin, 2012
Additional Reading Material:
Course/Module evaluation:
End of year written/oral examination 0 %
Presentation 0 %
Participation in Tutorials 0 %
Project work 0 %
Assignments 40 %
Reports 0 %
Research project 0 %
Quizzes 60 %
Other 0 %
Additional information:
The course consists of 4 hour during the semester and a laboratory work which will be concentrated in 5 days after the end of the semester (4-6 hours/day).
|
