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HU Credits:
2
Degree/Cycle:
1st degree (Bachelor)
Responsible Department:
Bio-Medical Sciences
Semester:
1st Semester
Teaching Languages:
Hebrew
Campus:
Ein Karem
Course/Module Coordinator:
Prof Joel Yisraeli
Coordinator Office Hours:
Contact by email to set up an appointment
Teaching Staff:
Prof Joel Yisraeli,
Prof Tally Naveh-Many,
Ms. Tirza Bidany,
Mr. Natan Florsheim,
Ms. Tehila Gershon,
Mr. Wajeeh Salaymeh
Course/Module description:
To expose students to molecular biology techniques by participating in an ongoing project to clone, characterize, and express a given gene
Course/Module aims:
To experience the thrill of molecular biology and to concretize subjects learned in a parallel lecture course on molecular biology
Learning outcomes - On successful completion of this module, students should be able to:
To perform basic techniques and strategies employed in standard molecular biology labs today:
1. DNA cloning
2. Restriction mapping
3. DNA and RNA analysis
4. PCR and RT-PCR
5. Basic bioinformatic analysis
6. Recombinant protein expression and analysis
Attendance requirements(%):
100%
Teaching arrangement and method of instruction:
Lab instruction
Course/Module Content:
Lab 1 – Introduction, preparing solutions
Preparing competent cells, ligation
Lab 2 – Extracting plasmid DNA and preparing for sequencing
Lab 3- Computer lab - NCBI searches, RNA comparisons, protein comparisons
Lab 4 – Computer lab - in silico restriction enzyme digestion and characterisation and transfer to an expression vector. Theoretical transfection into animal cells.
Lab 5 – Extracting RNA from HEK 293 cells and electrophoresis of RNA. Preparation of cDNA and analysis by PCR.
Lab 6 – Electrophoresis of PCR products. Protein gel electrophoresis and transfer.
Lab 7 – Western blot
Lab 8 - Summary seminars
Required Reading:
online lab manuals
Additional Reading Material:
Grading Scheme :
Active Participation / Team Assignment 10 %
Submission assignments during the semester: Exercises / Essays / Audits / Reports / Forum / Simulation / others 40 %
Mid-terms exams 20 %
Personal Guide / Tutor / Team Evaluation 10 %
Presentation / Poster Presentation / Lecture 20 %
Additional information:
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