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HU Credits:
2
Degree/Cycle:
2nd degree (Master)
Responsible Department:
Structural & Molecular Biochemistry
Semester:
2nd Semester
Teaching Languages:
English and Hebrew
Campus:
E. Safra
Course/Module Coordinator:
Dr. Hadar Amartely
Coordinator Office Hours:
By appointment
Teaching Staff:
Dr. Hadar Amartely,
Prof. Oded Livnah
Course/Module description:
Different methods in protein separation and purification, protein analysis and characterization and quantitative methods in protein interactions.
Course/Module aims:
Provide basic knowledge in protein separation for students that protein production and purification is necessary for their research.
Learning outcomes - On successful completion of this module, students should be able to:
1. Design a purification procedure for different proteins according to the properties of a protein, combining varied purification methods.
2. Choose and design analytical methods for protein characterization. 3. Design binding assays using different biophysical methods
Attendance requirements(%):
100%
Teaching arrangement and method of instruction:
Frontal
Course/Module Content:
Strategies in Protein purification and development of a protocol. Selection and combination of purification techniques.
Application of protein purification and scales of purification.
FPLC system, classical chromatography methods (e.g IEX, SEC, HIC, affinity and mixed mode). Basis for selectivity, optimization of purification procedure. Protein quality control and characterization:
Monitoring problems of aggregation. Protein refolding.
Methods for studying protein interactions.
Required Reading:
Selected papers and handbooks
Additional Reading Material:
None
Grading Scheme :
Additional information:
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