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Syllabus SEPERATION METHODS IN BIOLOGY - 73521
עברית
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Last update 29-01-2017
HU Credits: 2

Degree/Cycle: 2nd degree (Master)

Responsible Department: animal & veterinary science

Semester: 2nd Semester

Teaching Languages: Hebrew

Campus: Rehovot

Course/Module Coordinator: Orna Halevy

Coordinator Email: orna.halevy@mail.huji.ac.il

Coordinator Office Hours: By appointment

Teaching Staff:
Prof Orna Halevy

Course/Module description:
General knowledge of the theory behind chromatography. Study of methods for separation of micromolecules (e.g., vitamins, lipids) and macromolecules (e.g., proteins).

Course/Module aims:
General knowledge of the theory behind chromatography. Study of methods for separation of micromolecules (e.g., vitamins, lipids) and macromolecules (e.g., proteins).

Learning outcomes - On successful completion of this module, students should be able to:
Plan experiments including the chromatography methods
Determine purity level of protein
Solve problems of zone spreading
Solve problems of protein identification

Attendance requirements(%):
80%

Teaching arrangement and method of instruction: Frontal class

Course/Module Content:
Principles of chromatography,chromatography of macromolecules:
absorption,reverse-phase,ion exchange,gel filtration,affinity.
Electrophoresis according to charge,molecular
weight,iso-electrofocusing,Western blots,identification of
molecules,

Required Reading:
Articles

Additional Reading Material:
Articles, Websites

Course/Module evaluation:
End of year written/oral examination 100 %
Presentation 0 %
Participation in Tutorials 0 %
Project work 0 %
Assignments 0 %
Reports 0 %
Research project 0 %
Quizzes 0 %
Other 0 %

Additional information:
Recommended to first year MSc students
 
Students needing academic accommodations based on a disability should contact the Center for Diagnosis and Support of Students with Learning Disabilities, or the Office for Students with Disabilities, as early as possible, to discuss and coordinate accommodations, based on relevant documentation.
For further information, please visit the site of the Dean of Students Office.
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