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HU Credits:
5
Degree/Cycle:
1st degree (Bachelor)
Responsible Department:
Biochemistry & Food Sciences
Semester:
1st Semester
Teaching Languages:
Hebrew
Campus:
Rehovot
Course/Module Coordinator:
Prof. Oren Froy
Coordinator Office Hours:
Schedule ad hoc via email
Teaching Staff:
Mr. amit hamburg,
Ms. OR maalumi,
Ms. tali gorbonos,
Dr. Ido Goldstein,
Prof Oren Froy
Course/Module description:
The course consists of 2 parts that are complementary. The lectures are given 4 hours per week. Three additional hours are for exercises. There will 3 exercises
Course/Module aims:
1. Providing knowledge on the structure and function of biological molecules/compounds: proteins, fats and sugars.
2. Presenting the relationship between molecular structure of compounds and their function in the organism (enzymes, transporters, biological membranes)
3. Study their metabolic pathways : synthesis and degradation ; regulation and pathological-related processes
Learning outcomes - On successful completion of this module, students should be able to:
1.Describe the characteristics of the different levels of protein structure and how such characteristics relate to the amino acid sequence
2.Describe what an enzyme is and the basis of its function. The student should also be
able to understand basic enzyme kinetics and enzyme inhibition patterns of drugs
3.Describe simple and more complex lipids and some of their roles in living organisms membrans
4. Review the major classes of proteins involved in transport of small molecules across
membranes.
5.Outline how energy is harvested and used to drive cellular reactions
6.Describe and illustrate the metabolic pathways of glycolysis, glycogenolysis,
gluconeogenesis, the citric acid cycle, oxidative phosphorylation, the synthesis and
degradation of fatty acids and the synthesis of ketone bodies, The synthesis and
degradation of amino acids , Photosynthsis.
7. Identifiy the interrelationships between the various metabolic pathways and outline their overall regulation
Attendance requirements(%):
Mandatory only in exercises
Teaching arrangement and method of instruction:
Teaching in classroom
Course/Module Content:
• Proteins : Classification, chemical and physical properties, Bonds maintaining protein structure , organization of protein structure, alpha helix and beta pleated sheath .Globular proteins and fibrous proteins. Classification and chemical structures of amino acids, Chemical reactions of amino acids ; Isoelectric pH , acid base properties of amino acids, Structure , function and relationship of a protein : Haemoglobin and myoglobin
• Enzymes: classification of enzymes with examples .Apoenzyme , coenzyme, holoenzyme and cofactors, Kinetics of enzymes : Reaction velocity, order of reaction, specific activity, Michaelis-Menten equation .Significance of Km., Factors affecting enzymatic activity : temperature , pH , substrate concentration and enzyme concentration. Inhibitors of enzyme action : Competitive , non- competitive, irreversible, Enzyme : Mode of action, allosteric and covalent regulation, Functional and nonfunctional enzymes . Clinical significance of enzymes, Measurement of enzyme activity and interpretation of units. Measurement with coupled reactions.
• Membrane structure and transport: Membrane composition, Fluid mosaic model of Singer & Nicolson. Active & passive transport, Na-K pump, Calcium Pump
• General introduction to types of metabolic pathways and common strategies used in energy metabolism, bioenergetics.
• Overview of carbohydrate and lipid metabolism. Glycolysis, gluconeogenesis, glycogen metabolism and the regulation of these pathways, discussing hormonal, covalent and allosteric control of enzymes. The citric acid cycle and its central role in metabolism. Oxidative phosphorylation, electron transfer and ATP synthesis. Lipid metabolism. The mobilization of fats. Fatty acid catabolism. Ketone body formation. Fatty acid biosynthesis. Regulation of fatty acid and lipid metabolism.
• Integration and overall control of metabolism.
Required Reading:
-
Additional Reading Material:
1. Stryer, Biochemistry, 3rd ed.
2. Stryer, Biochemistry, 4th ed.
3. Stryer, Biochemistry, 5th ed.
4. Lehninger, Principles of Biochemistry, 2nd ed.
5. Lehninger, Principles of Biochemistry, 3rd ed.
Grading Scheme :
Submission assignments during the semester: Exercises / Essays / Audits / Reports / Forum / Simulation / others 52 %
Mid-terms exams 48 %
Additional information:
Final exam and quiz.
The course will be recorded but permission to watch will be given by Prof. Froy.
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